Protocols

Multiplexed scNOMe-seq protocol based on isolated single nuclei

This is the protocol we are currently using for our standard single cell Nucleosome Occupancy and Methylome sequencing (scNOMe-seq) assays. We adapted the single-cell methylome sequencing protocol described in Luo et al., 2018 for use in scNOMe-seq.

Protocol

Code

Drop-seq and 10x processing pipeline

This lightweight scRNA-seq processing pipeline works with both Drop-seq/DroNc-seq and 10x (v2/3) chemistry. The pipeline is developed and maintained by Alan Selewa.

scRNA-seq processing pipeline GitHub

A GitHub repository containing analysis from Selewa et al.

This repository contains the code for the analysis performed in Selewa et al.

Selewa et al. GitHub